Nguyen Le Anh Dao1,2, Guy Degand2, François Brose2, Tran Minh Phu1,Joëlle Quetin-Leclercq3, Bui ThiBuuHue4, Le ThiBach4, Truong Quynh Nhu1, Bui ThiBich Hang1, Do ThiThanh Huong1, Nguyen Thanh Phuong1, Patrick Kestemont5, Marie-Louise Scippo2
1College of Aquaculture & Fisheries, Can Tho University, Can Tho, Vietnam
2Department of Food Sciences,Faculty of Veterinary Medicine, University of Liège, Liège, Belgium
3Louvain Drug Research Institute, Pharmacognosy research group, Universitécatholique de Louvain, Brussels, Belgium
4College of Natural Sciences, CanTho University, Can Tho, Vietnam
5Department of biology, Faculty of Sciences,University of Namur, Namur, Belgium
This study was conducted to determine the in vitro antioxidant activities of 20 herbal extract samples and 3 commercial products for initial selection of potential application in seafood and aquaculture products storage. The methods of Folin-Ciocalteu (FC) and 2,2-diphenyl-1-pycrylhydrazyl radical scavenging (DPPH) were applied to evaluate the antioxidant activity. The results showed that eleven out of twenty three plant extracts displayed antioxidant activity, with concentrations able to inhibit half of the maximum response (IC50) ranging from 6 to 49 µg/mL.PhyllanthusamarusSchum. etThonn.showed the strongest radical scavenging effect (IC50=6 µg/mL). The remaining 4 samples showing ahigh antioxidant activity (i.e. IC50< 30 µg/mL) were in the following order: Piper betleL. >PsidiumguajavaL. >Euphorbia hirta L.>Mimosa pudicaL..Agroup of six samples,includingZingiberofficinaleRosc.,Hepamin, Ecliptaprostrata (L.)L., Alba, AnnonareticulataL., HouttuyniacordataThunb.,showed an intermediate antioxidant capacity (i.e30 µg/mL<IC50< 50 µg/mL). There was a positive correlation between total phenolic content (expressed as gallic acid equivalents) and antioxidant activity (expressed as 1/IC50) (R2=0.9137).
The next step will be to test compounds with high in vitro antioxidant activity for their capacity to inhibit fatty acid oxidation in feed used in aquaculture and aquaculture products.
Key words: Antioxidant activity, Folin-Ciocalteu, herbal extracts, plant extracts,2,2-diphenyl-1-pycrylhydrazyl radical scavenging.